4T1-EGFP cell line

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Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Cell Monoclonal Culture Medium

Monoclone Genotype Validation Kit

4T1-EGFP Order

Catalog#

YC-B004-EGFP-P

Size

1*10^6

Transfection Culture Medium

Transfection Culture medium-B

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Datasheet

Order

Detailed Product Information

Product category

EGFP Stable Cell Lines

cell name

4T1-EGFP

Morphology

Epithelial-like, adherent

Passage ratio

1:3~1:4

Species

Mouse

Reporter gene

EGFP

Resistance gene

puro

Construction method

Lentivirus

Mycoplasma test

negative

Culture method

88%RPMI-1640+10% FBS+1%Sodium Pyruvate+1%Glutamax

STR Profile

Loci	STR Info (Sample Cell) Sample Cell Line:4T1			STR Info (Cell bank) Cell Line:4T1
Loci	Allele1	Allele2	Allele3	Allele1	Allele2	Allele3
4-2	241.11 【21.3】			21.3
5-5	335.53 【14】			14.0
6-4	299.38 【18】			18.0
6-7	334.14 【12】			12.0
9-2	220.63 【15】			15.0
12-1	225.61 【16】			16.0
15-3	201.37 【22.3】			22.3
18-3	159.26 【18】	163.73 【19】		18.0	19.0
X-1	399.75 【25】			25.0
D4S2408

* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases

EGFP validation

Instruction

Use EGFP gene expressing lentivirus constructed by Ubigene to infect the 4T1 cell line， screen the cells based on the puromycin resistance marker and the optimal antibiotic screening concentration explored in advance, resulting in a 4T1-EGFP cell line that can maintain long-term, stable and high expression.

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